Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
Potassium Phosphate, 0.5 M Buffer Solution, pH 7.0, PROMO, Thermo Scientific Chemicals
CAS: 7778-77-0 Molecular Formula: H2KO4P Molecular Weight (g/mol): 136.08 g/mol MDL Number: MFCD00011401 MFCD00147253 InChI Key: GNSKLFRGEWLPPA-UHFFFAOYSA-M IUPAC Name: potassium dihydrogen phosphate SMILES: [K+].OP(O)([O-])=O
| CAS | 7778-77-0 |
|---|---|
| Molecular Weight (g/mol) | 136.08 g/mol |
| MDL Number | MFCD00011401 MFCD00147253 |
| SMILES | [K+].OP(O)([O-])=O |
| IUPAC Name | potassium dihydrogen phosphate |
| InChI Key | GNSKLFRGEWLPPA-UHFFFAOYSA-M |
| Molecular Formula | H2KO4P |
Sodium Cacodylate, 0.1 M Buffer Solution, pH 7.0, PROMO, Thermo Scientific™
CAS: 6131-99-3 Molecular Formula: C2H6AsNaO2 Molecular Weight (g/mol): 159.98 g/mol MDL Number: MFCD00149079 InChI Key: IHQKEDIOMGYHEB-UHFFFAOYSA-M IUPAC Name: sodium dimethylarsinate SMILES: [Na+].C[As](C)([O-])=O
| CAS | 6131-99-3 |
|---|---|
| Molecular Weight (g/mol) | 159.98 g/mol |
| MDL Number | MFCD00149079 |
| SMILES | [Na+].C[As](C)([O-])=O |
| IUPAC Name | sodium dimethylarsinate |
| InChI Key | IHQKEDIOMGYHEB-UHFFFAOYSA-M |
| Molecular Formula | C2H6AsNaO2 |
PIPES, 1.0 M Buffer Solution, pH 6.5, PROMO, Thermo Scientific Chemicals
CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 g/mol MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
| PubChem CID | 79723 |
|---|---|
| CAS | 5625-37-6 |
| Molecular Weight (g/mol) | 302.36 g/mol |
| ChEBI | CHEBI:44933 |
| MDL Number | MFCD00006159 |
| SMILES | C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O |
| IUPAC Name | 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid |
| InChI Key | IHPYMWDTONKSCO-UHFFFAOYSA-N |
| Molecular Formula | C8H18N2O6S2 |
MES, 1.0 M Buffer Solution, pH 9.0, Thermo Scientific™
CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 g/mol MDL Number: MFCD00283848 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O
| PubChem CID | 16218417 |
|---|---|
| CAS | 145224-94-8 |
| Molecular Weight (g/mol) | 213.25 g/mol |
| MDL Number | MFCD00283848 |
| SMILES | C1COCCN1CCS(=O)(=O)O.O |
| IUPAC Name | 2-morpholin-4-ylethanesulfonic acid;hydrate |
| InChI Key | MIIIXQJBDGSIKL-UHFFFAOYSA-N |
| Molecular Formula | C6H15NO5S |
TBE Running Buffer, 10X, PROMO, Thermo Scientific Chemicals
Commonly used in nucleic acid electrophoresis. TBE Buffer is used for polyacrylamide and agarose gel electrophoresis. It is ideally suited for DNA and RNA separation on longer run, higher voltage or amperage gels.
MOPS, 0.5 M Buffer Solution, pH 7.5, PROMO, Thermo Scientific Chemicals
CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 g/mol MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N PubChem CID: 70807 ChEBI: CHEBI:44115 IUPAC Name: 3-morpholin-4-ylpropane-1-sulfonic acid SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1
| PubChem CID | 70807 |
|---|---|
| CAS | 1132-61-2 |
| Molecular Weight (g/mol) | 209.26 g/mol |
| ChEBI | CHEBI:44115 |
| MDL Number | MFCD00006183 |
| SMILES | [O-]S(=O)(=O)CCC[NH+]1CCOCC1 |
| IUPAC Name | 3-morpholin-4-ylpropane-1-sulfonic acid |
| InChI Key | DVLFYONBTKHTER-UHFFFAOYSA-N |
| Molecular Formula | C7H15NO4S |
HEPES, Biological Buffer, 99%, Spectrum™ Chemical
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CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
| CAS | 7365-45-9 |
|---|---|
| Molecular Weight (g/mol) | 238.30 |
| MDL Number | MFCD00006158 |
| SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
| IUPAC Name | 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid |
| InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| Molecular Formula | C8H18N2O4S |
Bis-Tris Hydrochloride, Biological Buffer, 99%, Spectrum™ Chemical
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CAS: 124763-51-5 Molecular Formula: C8H20ClNO5 Molecular Weight (g/mol): 245.70 InChI Key: VEYRVLHAMHQVTC-UHFFFAOYSA-N IUPAC Name: 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol hydrochloride SMILES: Cl.OCCN(CCO)C(CO)(CO)CO
| CAS | 124763-51-5 |
|---|---|
| Molecular Weight (g/mol) | 245.70 |
| SMILES | Cl.OCCN(CCO)C(CO)(CO)CO |
| IUPAC Name | 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol hydrochloride |
| InChI Key | VEYRVLHAMHQVTC-UHFFFAOYSA-N |
| Molecular Formula | C8H20ClNO5 |
Tris(hydroxymethyl)aminomethane, Crystal, ACS, 99.8-100.1%, Spectrum™ Chemical
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CAS: 77-86-1 Molecular Weight (g/mol): 121.14 g/mol
| CAS | 77-86-1 |
|---|---|
| Molecular Weight (g/mol) | 121.14 g/mol |
Tris(hydroxymethyl)aminomethane, USP, EP, BP, bioCERTIFIED™, 50 kg, Spectrum Chemical
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Gibco™ PBS, pH 7.4
Gibco PBS, pH 7.4, is a balanced salt solution ideal for cell culture applications. It lacks calcium, magnesium, and phenol red, preventing cell adhesion and assay interference. Trusted for quality, Gibco PBS ensures reliable results in cell washing, transport, and reagent preparation.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Physical Form | Liquid |
|---|---|
| pH | 7.3 to 7.5 |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Recommended Storage | 15°C to 30°C |
| Concentration | 1 X |
| For Use With (Application) | Mammalian Cell Culture |
| Osmolality | 280 - 315 mOsm/kg |
| Sterility | Sterile-filtered |
Gibco™ DPBS, powder, calcium, magnesium
Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Includes | No Sodium Pyruvate |
|---|---|
| Physical Form | Powder |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | DPBS |
| For Use With (Application) | Mammalian Cell Culture |
| Solution Type | Dulbecco's Phosphate Buffered Saline |
| Product Line | Gibco™ |